101440
论文已发表
提 交 论 文
注册即可获取Ebpay生命的最新动态
注 册
IF 收录期刊
- 3.3 Breast Cancer (Dove Med Press)
- 3.4 Clin Epidemiol
- 2.5 Cancer Manag Res
- 2.9 Infect Drug Resist
- 3.5 Clin Interv Aging
- 4.7 Drug Des Dev Ther
- 2.7 Int J Chronic Obstr
- 6.6 Int J Nanomed
- 2.5 Int J Women's Health
- 2.5 Neuropsych Dis Treat
- 2.7 OncoTargets Ther
- 2.0 Patient Prefer Adher
- 2.3 Ther Clin Risk Manag
- 2.5 J Pain Res
- 2.8 Diabet Metab Synd Ob
- 2.8 Psychol Res Behav Ma
- 3.0 Nat Sci Sleep
- 1.8 Pharmgenomics Pers Med
- 2.7 Risk Manag Healthc Policy
- 4.2 J Inflamm Res
- 2.1 Int J Gen Med
- 4.2 J Hepatocell Carcinoma
- 3.7 J Asthma Allergy
- 1.9 Clin Cosmet Investig Dermatol
- 2.7 J Multidiscip Healthc
肺组织细胞外囊泡介导的 miR-128-3p 传递是急性肺部炎症的一种新机制
Authors Deng W , Zhu X, Li H, Hu P, Qian K, Liu F
Received 4 December 2024
Accepted for publication 5 April 2025
Published 15 April 2025 Volume 2025:20 Pages 4831—4848
DOI http://doi.org/10.2147/IJN.S510241
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Professor Eng San Thian
Wei Deng,1– 3 Xiaoping Zhu,3 Hang Li,4 Ping Hu,1,2 Kejian Qian,1,2 Fen Liu1,2
1Department of Critical Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, People’s Republic of China; 2Medical Innovation Center, First Affiliated Hospital of Nanchang University, Nanchang, People’s Republic of China; 3Department of Anesthesiology, The First Affiliated Hospital of Nanchang University, Nanchang, People’s Republic of China; 4Department of Dermatology, The First Affiliated Hospital of Nanchang University, Nanchang, People’s Republic of China
Correspondence: Fen Liu, Email liufen9934@163.com
Background: Emerging evidence links macrophage overactivation to sepsis-associated acute lung injury (ALI), yet the role of lung tissue-derived extracellular vesicles (Ti-EVs) in this process remains unclear. This study combines transcriptomic profiling and functional validation to reveal how Lung Ti-EVs mediate macrophage polarization through miRNA-dependent NLRP3 inflammasome activation.
Methods: We established a sepsis mouse model, extracted and characterized lung tissue-derived EVs, performed high-throughput transcriptome sequencing and bioinformatics analysis. Intratracheal administration of these EVs to wild-type C57BL/6 mice revealed their effects on pulmonary inflammation, macrophage polarization, and proliferation. In vitro co-culture experiments with Raw264.7 macrophages further validated these findings and explored underlying mechanisms.
Results: We identified extracellular vesicles (EVs) enriched in lung tissues from septic ALI mice, selectively carrying miRNAs including miR-128-3p. In vivo administration of these EVs exacerbated pulmonary inflammation by expanding M1 macrophage populations, while in vitro experiments demonstrated EV-mediated miR-128-3p delivery to macrophages stimulated TNF-α and IL-6 production. Mechanistically, miR-128-3p promoted macrophage proliferation and inflammatory responses by targeting Rab20.
Keywords: lung tissue extracellular vesicles, sepsis, acute lung inflammation, miR-128-3p, macrophages
